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WebReal-time reverse transcription and Western blot analyses were performed to verify ETV4 expression in TNBC cells and tissue. Immunohistochemistry was used to detect expression of ETV4 protein in 135 TNBC tissue samples for association between ETV4 protein expression and clinical outcomes. Results: A total total of eight upregulated (CCL7, KISS1 ... WebAdipose tissue lysis for western analysis Adipose tissue lysis for western analysis HNTG Lysis Buffer 5.96 g HEPES (50 mM) 4.38 g NaCl (150 mM) 50 mL glycerol (10%) 5 mL Triton X-100 (1%) Bring to a volume of 400 mL using Milli-Q water and pH to 7.5 using NaOH. Bring to a total volume of 500 mL. perks of turning 50
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