WebClick-iT® RNA Imaging Kits 6 3.3 Remove the fixative and wash each well once with PBS. 3.4 Remove the wash solution and add 1 mL of 0.5% Triton® X-100 in PBS to each well. Incubate for 15 minutes at room temperature. 3.5 Prepare a 1X working solution of Click-iT® reaction buffer additive by diluting the 10X solution (prepared in step 1.3, see Table 3 for … WebThis tool allows users to query gene expression from mouse or human kidney and human kidney organoid single cell datasets. Libraries were created with either cells or nuclei as indicated, and with either the InDrop, DropSeq, sci-RNA-seq or 10X Chromium platforms. For details of each dataset in KIT, please read the original paper or visit the ...
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