Hcrt cre
WebHere we used a new Hcrt-IRES-Cre mouse line that achieved ChR2 expression eutopically in Hcrt neurons by the endogenous Hcrt gene locus, combined with the powerful Cre-dependent EF1α promoter. This combination produces many more ChR2 molecules per neuron than the original 1.2 Kb mouse promoter packaged into lentiviral vectors . … Weborexin-Cre Molecular description: A 3.2 kb fragment of the 5' upstream region of the human HCRT gene drives expression of cre and eGFP. The eGFP gene is separated from the cre gene by an IRES element. Cre …
Hcrt cre
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WebImages for hypocretin ( Hcrt ) TRAP (translating ribosome affinity purification) founder line JD218 This line is available from The Jackson Laboratory, stock ID 028619 Click on a thumbnail of mouse brain to examine the image in detail. WebSelecting multiple terms will filter for results with any (i.e. logical OR) of the selected terms.
WebJul 23, 2024 · Hcrt-LH and LepRb-LH neurons: physiology and behavior. To establish selective Hcrt-LH targeting, we developed a knock-in IRES-Cre mouse line (Hcrt-Cre; Supplementary Fig. 1a,b).Following intra-LH ... WebApr 23, 2024 · Mice injected with AAV-HCRT-CRE showed glucose intolerance in the glucose tolerance test , as compared to mice injected with an AAV control virus (one-way ANOVA, t(AAV-HCRT-CRE, AAV-control) = −3.56676, p = 0.00442). This reinforces a regulatory role of IGF-I on orexin responses to glucose increases. 2.4. IGF-I Modulates …
Web023418 B6.Cg-Tg(HCRT-MJD)1Stak/J Hypocretin (orexin) containing neurons are ablated in these transgenic mice, which may be useful in studies of narcolepsy, regulation of … WebMay 1, 2024 · Using a Hcrt-Cre transgenic mouse line combined with optogenetic release of Hcrt in a temporal manner, we have shown antagonism of HcrtR2 mainly mediates the …
WebLentiviral vectors contained either Hcrt::ChR2-mCherry or Hcrt::mCherry sequences and were prepared as previously described . Cre-inducible recombinant AAV vectors carrying optogenetic transgenes were serotyped with AAV5 coat proteins and packaged by the viral vector core at the University of North Carolina.
WebHere we used a new Hcrt-IRES-Cre mouse line that achieved ChR2 expression eutopically in Hcrt neurons by the endogenous Hcrt gene locus, combined with the powerful Cre … blackhawk golf course menuWebJun 9, 2024 · C57BL/6J mice (4–6 months old) expressing GFP (Hcrt-GFP) (18, 38, 39, 44) or Cre-recombinase (Hcrt-Cre) selectively in hypocretin neurons under the control of a specific hypocretin promoter were used in this study. Mice were group housed and maintained on a 12-hour light/12-hour dark cycle (lights on at 6:00 am) with free access … games with big scytheWebresistant” CRE have been reported. • CRE have spread throughout most parts of the United States and other countries and have the potential to spread more widely. • Currently in … games with bitcoin rewardsWebJan 1, 2024 · Methods. Hcrt cre rats were generated using a CRISPR/Cas9-based approach. Viruses encoding optogenetic probes were injected into the perifornical lateral hypothalamic (PeFLH) area, optogenetic fibres were embedded in the PeFLH, BF, or LC, and changes in anaesthesia state under 1.4 vol% or 0.8 vol% isoflurane were determined. black hawk golf course richmond txWebFeb 19, 2015 · We employed two strategies for specifically expressing ChR2 in LHA LepRb neurons while visualizing Hcrt neurons for slice electrophysiology: (1) LepRb::cre mice were injected with a mixture of two ... blackhawk golf course janesville wisconsinWebJun 9, 2024 · Cre-recombinase (Hcrt-Cre) (82) selectively in hypocretin neurons under the control of a specific hypocretin promoter were used in t his study. Mice were group housed and maintained on a 12-12 hr blackhawk golf course wiWebSynergistic activation of CRH PVN and Hcrt LH neurons during restraint stress is consistent with our 155 observation of cFos staining as described in Fig. S1. 156 157 Mild optogenetic stimulation ... blackhawk golf course ohio sold