WebOct 5, 2015 · To test their effectiveness in zebrafish, we targeted 20 developmental genes and compared the morphological changes with mutant and morpholino (MO)-induced phenotypes. ASO-mediated transcript knockdown reproduced the published loss-of-function phenotypes for oep, chordin, dnd, ctnnb2, bmp7a, alk8, smad2 and smad5 in a dosage … WebChordin is an antagonist of the bone morpho- genetic protein (BMP) pathway copiously secreted by a dorsal region of the Xenopus embryo called “Spemann’s organizer” …
Chordin is required for the spemann organizer transplantation ...
WebA gene on chromosome 3q27 that encodes chordin, a secreted protein that dorsalises early vertebrate embryonic tissues by binding to ventralising TGF-beta-like bone … WebNov 1, 2010 · The morpholino sequence selected to target EGFP expression (EGFP-MO 0) has been shown to effectively knock down EGFP expression in both cell culture and in vivo. 31 The morpholino sequence selected to target the expression of endogenous zebrafish chordin (chordin-MO 0) routinely induces a specific morphological phenotype when … ppp loan 401k
Chordin Is Required for the Spemann Organizer ... - ResearchGate
WebMar 1, 2003 · We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin... WebMay 25, 2007 · DOI: 10.1371/journal.pgen.0030078 Abstract Morpholino phosphorodiamidate antisense oligonucleotides (MOs) and short interfering RNAs (siRNAs) are commonly used platforms to study gene function by sequence-specific knockdown. Both technologies, however, can elicit undesirable off-target effects. WebOct 1, 2015 · Overexpression of admp1 but not of admp2 in a chordin morpholino background caused 100% of the embryos to develop with a Nodal loss-of-function phenotype. (c) ... hans kelsen positivismo juridico